Bacillus subtilisα-amylase: the rate limiting step of secretion is growth phase-independent
نویسندگان
چکیده
منابع مشابه
Characterization of the rate-limiting step of the secretion of Bacillus subtilis alpha-amylase overproduced during the exponential phase of growth.
The Bacillus subtilis alpha-amylase gene, amyE, was expressed under the regulated control of sacR, the levansucrase leader region. The gene fusion including the complete amyE coding sequence with the signal peptide sequence was integrated into the chromosome of a degU32(Hy) strain deleted of the sacB DNA fragment. In this genetic contex, alpha-amylase is produced in the culture supernatant at a...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1999
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1999.tb13493.x